Syed Shams Yazdani

Home  >>  Syed Shams Yazdani

profile1

Syed Shams Yazdani – PhD

Group Leader- Microbial Engineering, Integrative Biology group
Coordinator- DBT-ICGEB Advanced Bioenery Research
International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, 110 067 New Delhi, India.
E-mail: shams@icgeb.res.in
To know more, please click on ICGEB Webpage

Research Interests

> Metabolic engineering, cellulolytic enzymes, biofuels.

Education

> Jawaharlal Nehru University, New Delhi, India, PhD (Biotechnology), 2000
> Jawaharlal Nehru University, New Delhi, India, MSc (Biotechnology), 1994
> Aligarh Muslim University, Aligarh, India, BSc (Hons) Chemistry, 1992

Career History

>  Since 2012, Coordinator of the DBT-ICGEB Centre for Advanced Bioenery Research,
International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi, India
>  Since 2011, Group Leader, Synthetic Biology and Biofuel Group, ICGEB, New Delhi
>  2007-2008, Postdoctoral Associate, Department of Chemical and Biomolecular Engineering, Rice
University, Houston, USA
>  2003-2010, Staff Research Scientist, Malaria Group, ICGEB, New Delhi
>  1999-2003, Research Scientist, Malaria Group, ICGEB, New Delhi

Scientific Activity

In our effort to develop cost effective process to produce second generation biofuels, we are isolating novel enzymes with higher specificities towards cellulosic biomass. We have identified novel cellulase and xylanase enzymes from bacteria isolated from mid-gut of insects living on agricultural biomass and overexpressed them in E. coli for use in saccharification process. Based on structural modelling, we have synthesized two chimeric bifuctional enzymes, one with the fusion between endocellulase and xylanase and second with the fusion between endocellulase and beta-glucosidase. Both these bifuctional enzymes were shown to be equal or more effective than their individual counterparts. We are now developing processes to produce these enzymes in the bioreators and testing their stability and activity at pilot scale.

We discovered sometime back a pathway in Escherichia coli that enables this organism to produce bio-ethanol from crude glycerol generated as waste from biodiesel industry. This work was performed in collaboration with Rice University, Houston, Texas. Further metabolic engineering of E. coli was done to enhance the yield of bioethanol. We are now working on production of bioethanol from pretreated lignocellulosic biomass with the help of metabolic engineering and system biology approaches to bring down costs. We are secreting various cellulytic enzymes in E. coli to enable it to utilize pretreated lignocellulosic biomass. In order to produce high level of ethanol using metabolic engineering tools, we are engineering E. coli to enhance bioethanol yield by blocking side pathways that produce competing co-products and by providing an alternate pathway that can fulfil NADH requirement for homo-ethanol production. In addition, we have identified few natural bacteria from guts of insect (living on plants) that degrade lignocellulosic biomass with high efficiency and are exploring the possibility of engineering these bacteria to produce bioethanol from lignocellulosic biomass. The development of an integrated biocatalyst that can perform both functions, i.e., conversion of complex cellulose and hemicellulose into monomeric sugar molecules and fermentation of monomeric sugar into biothanol, is likely to bring down the production cost of lignocellulosic ethanol considerably. We are also engineering a laboratory bacterium that can produce butanol, an alcohol that has properties closer to natural petroleum, from agricultural biomass.

 

Research Description

Discovery and design of novel enzymes and enzyme systems for biofuelsIn our effort to develop cost effective process to produce second generation biofuels, we are isolating novel enzymes with higher specificities towards cellulosic biomass. We have identified novel cellulase and xylanase enzymes from bacteria isolated from mid-gut of insects living on agricultural biomass and overexpressed them in E. coli for use in saccharification process. Based on structural modelling, we have synthesized two chimeric bifuctional enzymes, one with the fusion between endocellulase and xylanase and second with the fusion between endocellulase and beta-glucosidase. Both these bifuctional enzymes were shown to be equal or more effective than their individual counterparts. We are now developing metagenomic, metatranscriptomic and metaproteomic techniques to identify new cellulolytic enzymes from gut microbes.

Engineering bacteria to produce biofuel We are working on production of bioethanol from pre-treated lignocellulosic biomass with the help of metabolic engineering and system biology approaches to bring down costs. We are secreting various cellulytic enzymes in E. coli to enable it to utilize pretreated lignocellulosic biomass. In order to produce high level of ethanol using metabolic engineering tools, we are engineering E. coli to enhance bioethanol yield by blocking side pathways that produce competing co-products and by providing an alternate pathway that can fulfil NADH requirement for homo-ethanol production. By optimizing the expression of native pathway and without using any foreign gene, we show production of ethanol with high yield and productivity. In addition, we have identified few natural bacteria from guts of insect (living on plants) that degrade lignocellulosic biomass with high efficiency and are exploring the possibility of engineering these bacteria to produce bioethanol from lignocellulosic biomass. The development of an integrated biocatalyst that can perform both functions, i.e., conversion of complex cellulose and hemicellulose into monomeric sugar molecules and fermentation of monomeric sugar into biothanol, is likely to bring down the production cost of lignocellulosic ethanol considerably. We are also engineering laboratory bacteria that can produce butanol and alkane/alkene from agricultural biomass.

Publications

>  Ogunmolu FE, Jagadeesha NB, Kumar R, Kumar P, Gupta D, Yazdani SS. (2017) Comparative insights into the saccharification potentials     of a relatively unexplored but robust Penicillium funiculosum glycoside hydrolase 7 cellobiohydrolase. Biotechnology and Biofuels doi:     10.1186/s13068-017-0752 Link

>  Jawed K, Mattam AJ, Fatma Z, Wajid S, Abdin MZ, Yazdani SS (2017) Engineered Production of Short Chain Fatty Acid in Escherichia coli     Using Fatty Acid Synthesis Pathway. PLoS One. doi: 10.1371 / journal. pone. 0160035. eCollection 2016.Link

>  Fatma, Z., Jawed, K., Mattam, A.J, and Yazdani, S.S (2016) Identification of long chain specific aldehyde reductase and its use in enhanced      fatty alcohol production in E. coli. Metabolic Engineering 37:35–45 PubMed link

>  Chitnis CE, Mukherjee P, Mehta S, Yazdani SS, Dhawan S, Shakri AR, Bharadwaj R, Gupta PK, Hans D, Mazumdar S, Singh B, Kumar S,     Pandey G, Parulekar V, Imbault N, Shivyogi P, Godbole G, Mohan K, Leroy O, Singh K, Chauhan VS. (2015) Phase I Clinical Trial of a     Recombinant Blood Stage Vaccine Candidate for Plasmodium falciparum Malaria Based on MSP1 and EBA175. PLoS One. 2015 Apr     30;10(4):e0117820 PubMed link

>  Shakeel T, Fatma Z, Fatma T, Yazdani SS. (2015) Heterogeneity of alkane chain length in freshwater and marine cyanobacteria. Frontiers in     Bioengineering and Biotechnology 16;3:34. PubMed link

>  Munjal N, Jawed K, Wajid S, Yazdani SS. (2015) A Constitutive Expression System for Cellulase Secretion in Escherichia coli and Its Use in     Bioethanol Production. PLoS One 10(3):e0119917. PubMed link

>  Adlakha, N., Yazdani, S.S. (2015) Efficient production of (R,R)-2,3-butanediol fromcellulosic hydrolysate using Paenibacillus     polymyxa ICGEB2008. Journal of Industrial Microbiology and Biotechnology 42, 21-8. PubMed link

Patents

1. 4260/DEL/2015 Method for enhanced fatty alcohol production in E. coliInventor(s), Yazdani SS, Fatma, Z
2. WO2014033759A1 Method for enhanced fatty alcohol production in E. coliInventor(s), Yazdani SS, Fatma, Z
3. CN104838005A Method for enhanced fatty alcohol production in E. coliInventor(s), Yazdani SS, Fatma, Z
4. US2014424037A Method for enhanced fatty alcohol production in E. coliInventor(s), Yazdani SS, Fatma, Z
5. 1714/DEL/2015 A method for obtaining a composition for biomass hydrolysis. Inventor(s) Yazdani SS, Funso E
6. 2651/DEL/2013 Engineering E.coli strain for conversion of short chain fatty aids to bioalcohols. Inventor(s), Yazdani, S.S., Mattam, A.J.
7. 2695/DEL/2012 Novel cellulolytic enzymes and their chimera for hydrolysis of lignocellulosic biomass. Inventor(s),Yazdani SS, Adlakha N.
8. 2696/DEL/2012 Modified bacteria for the production of bioalcohols Inventor(s), Yazdani, S.S., Munjal, N., Mattam, A.J.
9. WO2017006352A2 A method for obtaining a composition for biomass hydrolysis. Inventor(s), Yazdani SS, Funso E

GROUP MEMBERS


profile2
Anmol Randhawa – PhD (Biological Sciences)

Research Associate
E-mail : anmolwareh@gmail.com.
Hobby: Capturing memorable moments in writings and poems.
Area of work: Fungal Expression Systems.We are exploring different ways & playing with different vector backgrounds, promoters and transformation systems to increase cellulolytic enzymes expression in fungi.

profile9
Tabinda Shakeel – PhD (Biological Sciences)

Research Associate
E-mail : tabinda2419@gmail.com.
Hobby: Restoring and reliving the visions of my peregrinations in the form of paintings and poetry.
Area of work: Engineering of microbial hosts for improved hydrocarbon production. We are currently exploring different tolerance mechanisms and novel gene targets for enhanced production of long chain alka(e)nes and alcohols.

profile6
Ali Samy Abdelaal – PhD (Genetics)

Postdoctoral Research Fellow
E-mail : asamy82@gmail.com.
Hobby: Traveling and exploring new cultures.
Area of work: Metabolic engineering of bacteria for alcohol production. Currently, I am working on ‘engineering of E. coli to produce ethanol/ or butanol from different carbon sources’

profile10
Tulika Sinha – M.sc (Biotechnology)

PhD Student
E-mail : tulika@icgeb.res.in.
Hobby: Listening to music, sightseeing, playing badminton
Area of work: Overproduction of cellulolytic enzymes in fungi. Currently, construing the role of transcription factors for cellulase overproduction.

profile4
Syed Bilal Jilani -M.Sc.(Biochemistry & Environmental Sciences)

Junior Research Fellow
E-mail : sbjilani@icgeb.res.in.
Hobby: Being outdoors, occasionally cooking food and reading non-fiction.
Area of work: Ethanologenic microbes, hydrolysate inhibition, stress biology.My goal is to identify key metabolic processes which when engineered into ethanologenic microbial strains shall result in relatively higher yield of industrially important metabolic products in presence of stressors across different biomass hydrolysate samples.

profile3
Olusola Ogunyewo B.Tech, M.Tech (Applied Biochemistry)

PhD Student
E-mail : akintayo@icgeb.res.in
Hobby: Travelling and listening to music.
Area of work: Fungal and Molecular Biology. Presently I am working on engineering the secretome of a hypercellulolytic fungus for enhanced saccharification of cellulosic biomass.

profile5
Raubins Kumar – M.sc (Biomedical Science)

PhD Student
E-mail : raubins@icgeb.res.in.
Hobby: Playing cricket, Visiting Historical and Natural places
Area of work: Metabolic Engineering of microbial host for short chain alcohols production

profile5
Chandra Dev – M.sc (Biotechnology)

PhD Student
E-mail : chandradevf16@gmail.com.
Hobby: Sketching, Admiring the Aviation.
Area of work: Rational redesigning of microbial genetic circuitry for efficient utilization of mixture of sugar. Currently we are focusing on co-utilization of glucose and xylose. This kind of strain can be of great industrial importance where the substrate is the mixture of sugars.

Careers at ICGEB

POSITIONS AVAILABLE

Applications invited RESEARCH ASSOCIATE AND JRF/SRF POSITIONS . Apply Soon..

Click here to know more

Post Resume